m 6 a lncrna epitranscriptomic microarrays  (Arraystar inc)

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway

doi: 10.1007/s00018-025-05594-z

Figure Lengend Snippet: SCIRT m 6 A modification and expression levels were elevated in NSCLC. ( a ) Heatmap of differentially m 6 A-methylated lncRNAs. ( b, c ) Differentially m 6 A-methylated lncRNA-related mRNAs were analyzed by GO and the KEGG using TCGA data. ( d ) SCIRT, RP11-385J1.2, and SNHG9 m 6 A methylation levels in 10 paired NSCLC and adjacent noncancerous tissues. ( e ) Relative expression levels of SCIRT in 10 paired NSCLC and adjacent noncancerous tissues. ( f ) SCIRT expression levels of NSCLC (A549, H1299, and H358) and bronchial epithelial cell lines (BEAS-2B). Data are presented as mean ± SD from at least three independent experiments. ** P < 0.01, *** P < 0.001

Article Snippet: Arraystar m 6 A lncRNA epitranscriptomic microarrays were used to screen differentially m 6 A-methylated lncRNAs in NSCLC, and array figures were shown in Supplementary Fig. . A total of 39 differential metylated candidates were identified with 24 upregulated genes and 15 downregulated genes (Table ).

Techniques: Modification, Expressing, Methylation

Journal: Clinical Epigenetics

Article Title: Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m 6 A-modified long non-coding RNAs in lung adenocarcinoma

doi: 10.1186/s13148-023-01475-z

Figure Lengend Snippet: Characteristics of the m 6 A-modified lncRNA microarray in LUAD tumor tissues and adjacent normal tissues. A The source distribution of m 6 A-modified lncRNA transcripts. B The length distribution of m 6 A-modified lncRNA transcripts (≤ 3000 bp). C The chromosomal localization of m 6 A-modified lncRNA transcripts. D Volcano plot showed that in 2846 m 6 A-modified lncRNA transcripts, 143 were differentially modified (|log 2 FC|> 0.585, P < 0.05, unpaired t test), and 123 were hypermethylated and 20 were hypomethylated. E The methylation level heatmap of 143 differentially modified lncRNA transcripts. F Volcano plot showed that in 143 differentially modified lncRNA transcripts, 32 were upregulated and 48 were downregulated (|log 2 FC|> 1, P < 0.05, unpaired t test). G The expression level heatmap of 143 differentially modified lncRNA transcripts

Article Snippet: Importantly, the Arraystar Human M 6 A-modified LncRNA Epitranscriptomic Microarray was utilized to analyze the characteristics of m 6 A-modified lncRNAs and screen out lncRNAs with differential methylation level in LUAD.

Techniques: Modification, Microarray, Methylation, Expressing

Journal: Clinical Epigenetics

Article Title: Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m 6 A-modified long non-coding RNAs in lung adenocarcinoma

doi: 10.1186/s13148-023-01475-z

Figure Lengend Snippet: Combined analyses of m 6 A modification and expression profiles of 143 differentially m 6 A-modified lncRNA transcripts in LUAD tissues. A The UpSet diagram was used to divide lncRNAs into six categories. B Spearman correlation analyses between the expression levels and methylation levels of 143 lncRNA transcripts in normal tissues (left), tumor tissues (middle), and all tissues (right). A point’s horizontal and vertical coordinates were the mean m 6 A level and mean expression level of a lncRNA transcript in six normal tissues (left), four tumor tissues (middle), and all ten tissues (right), respectively. C Validation of four lncRNAs’ expression levels in six pairs of clinical tissues; GAPDH served as the reference gene. * P < 0.05 and not significant (ns) P > 0.05. Paired t test. D Validation of four lncRNAs’ m 6 A levels in six pairs of clinical tissues by MeRIP-qPCR assays; Input was used as the reference. * P < 0.05, ** P < 0.01 and *** P < 0.001. Paired t test

Article Snippet: Importantly, the Arraystar Human M 6 A-modified LncRNA Epitranscriptomic Microarray was utilized to analyze the characteristics of m 6 A-modified lncRNAs and screen out lncRNAs with differential methylation level in LUAD.

Techniques: Modification, Expressing, Methylation, Biomarker Discovery

Journal: Clinical Epigenetics

Article Title: Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m 6 A-modified long non-coding RNAs in lung adenocarcinoma

doi: 10.1186/s13148-023-01475-z

Figure Lengend Snippet: The screening of prognostic m 6 A-regulated lncRNAs. A The screening criteria of 215 m 6 A-regulated lncRNAs and its expression correlation with m 6 A regulators. B Forest plot of univariate Cox regression analysis of m 6 A-regulated lncRNAs ( P < 0.01). The hazard ratio (HR) value, its 95% confidence interval (CI), as well as the associated P value, were shown. HR > 1 indicated that the lncRNA was a risk factor and its high expression was unfavorable for prognosis, while the high expression of the protective lncRNA of HR < 1 was favorable for prognosis. C The expression correlation between 13 prognostic m 6 A-regulated lncRNAs and m 6 A regulators

Article Snippet: Importantly, the Arraystar Human M 6 A-modified LncRNA Epitranscriptomic Microarray was utilized to analyze the characteristics of m 6 A-modified lncRNAs and screen out lncRNAs with differential methylation level in LUAD.

Techniques: Expressing

Journal: Clinical Epigenetics

Article Title: Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m 6 A-modified long non-coding RNAs in lung adenocarcinoma

doi: 10.1186/s13148-023-01475-z

Figure Lengend Snippet: Construction of m 6 A-induced ceRNA Network. A The subcellular localization of 43 differentially m 6 A-modified lncRNAs according to RNALoate. B The construction process of the ceRNA network, which consisted of 11 correlative m 6 A regulators, seven differentially modified lncRNAs, 30 sponged miRNAs, and 110 targeted mRNAs. C The diagram of the ceRNA network. The lines connecting m 6 A regulators and lncRNAs represented RNA co-expression in TCGA-LUAD set (| r |> 0.3 and P < 0.001), and the red line represented the lncRNA-m 6 A regulator protein combination detected by CLIP-seq recorded in the POSTAR3 database. D The chart emphasized the dysregulated targeted mRNAs in LUAD, whose numbers of connecting nodes were greater than or equal to 3 in the ceRNA network. FC represented the expression fold change of T-median/N-median. WilcoxTest. *** P < 0.001

Article Snippet: Importantly, the Arraystar Human M 6 A-modified LncRNA Epitranscriptomic Microarray was utilized to analyze the characteristics of m 6 A-modified lncRNAs and screen out lncRNAs with differential methylation level in LUAD.

Techniques: Modification, Expressing

Journal: Scientific Reports

Article Title: Human m 6 A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease

doi: 10.1038/s41598-021-99867-9

Figure Lengend Snippet: The differentially expressed profile of m6A-mRNAs in immature red blood cells of Hb CS thalassemia (T) and healthy volunteers controls (N) (* P < 0.05.). Relative mRNA expression, as evidenced by qRT-PCR. The qRT-PCR data ( A ) was consistent with the epitranscriptomic rnicroarray sequence ( B ).

Article Snippet: We conducted the Arraystar Human m 6 A-mRNA and lncRNA epitranscriptomic microarray analysis of 5 pairs of immature erythrocytes, particularly, 5 from HbH-CS thalassemia (T) and 5 from healthy volunteers (N).

Techniques: Expressing, Quantitative RT-PCR, Sequencing

Journal: Scientific Reports

Article Title: Human m 6 A-mRNA and lncRNA epitranscriptomic microarray reveal function of RNA methylation in hemoglobin H-constant spring disease

doi: 10.1038/s41598-021-99867-9

Figure Lengend Snippet: The 20 differentially hypo-methylated m6A other RNAS including lncRNA and other small RNAs.

Article Snippet: We conducted the Arraystar Human m 6 A-mRNA and lncRNA epitranscriptomic microarray analysis of 5 pairs of immature erythrocytes, particularly, 5 from HbH-CS thalassemia (T) and 5 from healthy volunteers (N).

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: M 6 A modification profiles of lncRNA& mRNA in LPS and control group. (A) Volcano plots showing the lncRNAs that were differentially methylated between LPS and control group with statistical significance. (B) Volcano plots showing the mRNAs that were differentially methylated between LPS and control group with statistical significance (fold changes ≥1.5 and p < 0.05). (C) Hierarchical clustering analysis the differentially methylated lncRNAs. (D) Hierarchical clustering analysis the differentially methylated mRNAs. Ctrl, control; LPS, lipopolysaccharide.

Article Snippet: Probes specific for 27,770 mRNAs and 10,582 lncRNAs were used to analyze the samples from the LPS and control groups using an m 6 A mRNA and lncRNA Arraystar epitranscriptomic microarray.

Techniques: Modification, Control, Methylation

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: The detailed information of the hyper-methylated and hypo-methylated lncRNAs.

Article Snippet: Probes specific for 27,770 mRNAs and 10,582 lncRNAs were used to analyze the samples from the LPS and control groups using an m 6 A mRNA and lncRNA Arraystar epitranscriptomic microarray.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: M 6 A single-base site qPCR was used to confirm the microarray data for the top four methylated lncRNAs and top six methylated mRNAs in left ventricle tissue between the LPS and control groups. * p < 0.05 versus the Ctrl group. Ctrl, control; LPS, lipopolysaccharide.

Article Snippet: Probes specific for 27,770 mRNAs and 10,582 lncRNAs were used to analyze the samples from the LPS and control groups using an m 6 A mRNA and lncRNA Arraystar epitranscriptomic microarray.

Techniques: Microarray, Methylation, Control

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: LncRNA XR_346,771-miRNA-mRNA ceRNA analysis. (A) The ceRNA network of XR_346,771–miRNA–mRNA. Red circles represent miRNAs, blue circles represent mRNAs, and green circles represent lncRNAs. (B) Ten most enriched GO terms involved in the ceRNA network. (C) KEGG pathways involved in the ceRNA network. ceRNA, competing endogenous RNA.

Article Snippet: Probes specific for 27,770 mRNAs and 10,582 lncRNAs were used to analyze the samples from the LPS and control groups using an m 6 A mRNA and lncRNA Arraystar epitranscriptomic microarray.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Lipopolysaccharide Alters the m6A Epitranscriptomic Tagging of RNAs in Cardiac Tissue

doi: 10.3389/fmolb.2021.670160

Figure Lengend Snippet: LncRNA XR_346771targeted miRNAs and mRNAs.

Article Snippet: Probes specific for 27,770 mRNAs and 10,582 lncRNAs were used to analyze the samples from the LPS and control groups using an m 6 A mRNA and lncRNA Arraystar epitranscriptomic microarray.

Techniques: